Figure 6, right, shows a small 5 ghz slice of the o1 site inhomogeneous line centered. For spectroscopy purposes, we choose to characterize light in the ultraviolet and visible regions in terms of wavelength expressed in nanometers. With this workbook users can deepen the theoretical knowledge they may already have gained and complement it with practical. Good laboratory practice with a uvvisible spectroscopy system application note uvvisible spectroscopy anthony j. Ultravioletvisible spectroscopy utrecht university repository. Mod02 lec06 ultraviolet and visible spectrophotometry 2 ii. Basic uvvis theory, concepts and applications uni salzburg. Since its development in the 1950s the uvvisible spectrophotometer. Ultravioletvisible spectroscopy i spectroscopy is based, principally, on the study of the interaction between radiation and matter. Owen the objective of good laboratory practice glp is to obtain accurate and precise results. Mod06 lec02 uvvisible spectroscopy video lecture by. Mod06 lec02 uvvisible spectroscopy video dailymotion.
Composition, construction limit components to finite useful wavelength ranges. Uv light can be absorbed by molecules to excite higher energy most loosely bound electrons from lower energy states to higher states. The transitions that result in the absorption of electromagnetic radiation in this region. In uvvisible spectroscopy, the lowwavelength uv light has the highest energy. The split beam approach to uvvis spectroscopy uses a. Uncertainty sources in uvvis spectrophotometric measurement. Mod06 lec02 uvvisible spectroscopy tutorial of analytical technologies in biotechnology course by prof ashwani k sharma of iit roorkee. A prerequisite for achieving this objective is a properlyfunctioning measurement system that is welldocumented. In some cases, this energy is sufficient to cause unwanted photochemical reactions when measuring sample spectra remember, it is the uv component of light that causes sunburn. Mod06 lec03 infrared and fluorescence spectroscopy. Mod06 lec05 nuclear magnetic resonance nmr spectroscopy and xray crystallography.
Instrumentation components may not at typically are not useful for all wavelength ranges. Mod06 lec02 uvvisible spectroscopy video lecture by prof. In the present chapter, uvvis and infrared spectroscopy have been discussed. Good laboratory practice with a uvvisible spectroscopy. Basic uv vis theory, concepts and applications page 6 of 28 figure 6 vapor and solution spectra of benzene general chemical origins when white light falls upon a sample, the light may be totally reflected, in which case the substance appears white or the light. Analytical technologies in biotechnology online course. Ultraviolet and visible spectroscopy this absorption spectroscopy uses electromagnetic radiations between 190 nm to 800 nm and is divided into the ultraviolet uv, 190400 nm and visible vis, 400800 nm regions. Ultraviolet spectroscopy ch521courseoninterpretavemolecular spectroscopy. Absorption of light in the uvvisible part of the spectrum 210 900 nm. This interaction causes in the atom an electronic transition from a lower energetic level, m, to a higher level, l, occurring energy absorption from the atom equal to the energy difference between both levels, e.
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